Chronic caffeine use does not influence behavior and brain oxidative status in mice

O uso crônico de cafeína não influencia o comportamento e o status oxidativo em camundongos

Abstract

The present study investigated whether and to what extent caffeine chronic use can influence the brain oxidative status and the behavioral activity of the C57BL/6 female mice. For this, fifteen animals were randomized in the following groups: Control, Caf10: 10 mg/kg caffeine, and Caf50: 50 mg/kg caffeine. The animals received one daily caffeine dose by i.p route by 120 days. Twenty-four hours after the last administration, the animals were subjected to behavioral tests and euthanized. The blood was used by biochemical analysis and the brain to evaluate the oxidative status and micromineral levels. The caffeine does not influence the anthropometric parameters, lipid profile, and the C-reactive protein levels. In the same way, SOD and glutathione-s-transferase (GST) activities maintained the same response profile. On the other hand, catalase (CAT) activity was decreased in both groups receiving caffeine. Despite this, malondialdehyde and carbonyl protein levels did not change among the groups, as well as the distribution of the chemical elements. In the same way, none caffeine dose was caused increased anxiety degree in the animals. Considering the time of caffeine administration, we believe that there was the cellular adaptation triggered by its use, tending to a protective effect on the brain.